Journal: Microorganisms

Article Title: Development of Polymerase Chain Reaction–High-Resolution Melt Assay for Waterborne Pathogens Legionella pneumophila , Vibrio parahaemolyticus , and Camplobacter jejuni

doi: 10.3390/microorganisms12071366

Figure Lengend Snippet: Bacterial DNA utilized for specificity testing.

Article Snippet: Bacillus cereus , str. NRRL B-568 , ATCC (10876D-5).

Techniques:

Journal: Bioinformatics and Biology Insights

Article Title: Functional Annotation Analytics of Bacillus Genomes Reveals Stress Responsive Acetate Utilization and Sulfate Uptake in the Biotechnologically Relevant Bacillus megaterium

doi: 10.4137/BBI.S7977

Figure Lengend Snippet: Bacillus cereus strains with finished genome sequences.

Article Snippet: The Invitrogen Oligo Perfect system was used to design primers to genes encoding two B. cereus USPs ZP04319786 and ZP04315896 as well as tuf gene, encoding elongation factor Tu.

Techniques:

Journal: Bioinformatics and Biology Insights

Article Title: Functional Annotation Analytics of Bacillus Genomes Reveals Stress Responsive Acetate Utilization and Sulfate Uptake in the Biotechnologically Relevant Bacillus megaterium

doi: 10.4137/BBI.S7977

Figure Lengend Snippet: Accesion numbers and locus tags of universal stress proteins in selected Bacillus cereus genomes.

Article Snippet: The Invitrogen Oligo Perfect system was used to design primers to genes encoding two B. cereus USPs ZP04319786 and ZP04315896 as well as tuf gene, encoding elongation factor Tu.

Techniques:

Journal: Bioinformatics and Biology Insights

Article Title: Functional Annotation Analytics of Bacillus Genomes Reveals Stress Responsive Acetate Utilization and Sulfate Uptake in the Biotechnologically Relevant Bacillus megaterium

doi: 10.4137/BBI.S7977

Figure Lengend Snippet: Function of adjacent genes of universal stress protein (140 aa) in selected Bacillus cereus genomes.

Article Snippet: The Invitrogen Oligo Perfect system was used to design primers to genes encoding two B. cereus USPs ZP04319786 and ZP04315896 as well as tuf gene, encoding elongation factor Tu.

Techniques:

Journal: Bioinformatics and Biology Insights

Article Title: Functional Annotation Analytics of Bacillus Genomes Reveals Stress Responsive Acetate Utilization and Sulfate Uptake in the Biotechnologically Relevant Bacillus megaterium

doi: 10.4137/BBI.S7977

Figure Lengend Snippet: Function of adjacent genes of universal stress protein (152 aa) in selected Bacillus cereus genomes.

Article Snippet: The Invitrogen Oligo Perfect system was used to design primers to genes encoding two B. cereus USPs ZP04319786 and ZP04315896 as well as tuf gene, encoding elongation factor Tu.

Techniques:

Journal: Bioinformatics and Biology Insights

Article Title: Functional Annotation Analytics of Bacillus Genomes Reveals Stress Responsive Acetate Utilization and Sulfate Uptake in the Biotechnologically Relevant Bacillus megaterium

doi: 10.4137/BBI.S7977

Figure Lengend Snippet: Multi-genome alignment of gene neigbhorhood of genes for universal stress protein representative Bacillus cereus genomes. Notes: Universal stress protein (USP) gene in blue with diagonal lines. Bacillus cereus Q1 is only genome with transcriptional regulator of the ArsR family. Source of image: http://biocyc.org/ .

Article Snippet: The Invitrogen Oligo Perfect system was used to design primers to genes encoding two B. cereus USPs ZP04319786 and ZP04315896 as well as tuf gene, encoding elongation factor Tu.

Techniques:

Journal: Bioinformatics and Biology Insights

Article Title: Functional Annotation Analytics of Bacillus Genomes Reveals Stress Responsive Acetate Utilization and Sulfate Uptake in the Biotechnologically Relevant Bacillus megaterium

doi: 10.4137/BBI.S7977

Figure Lengend Snippet: Ligand binding sites for 140 aa universal stress proteins of selected Bacillus cereus strains.

Article Snippet: The Invitrogen Oligo Perfect system was used to design primers to genes encoding two B. cereus USPs ZP04319786 and ZP04315896 as well as tuf gene, encoding elongation factor Tu.

Techniques: Ligand Binding Assay, Binding Assay

Journal: Bioinformatics and Biology Insights

Article Title: Functional Annotation Analytics of Bacillus Genomes Reveals Stress Responsive Acetate Utilization and Sulfate Uptake in the Biotechnologically Relevant Bacillus megaterium

doi: 10.4137/BBI.S7977

Figure Lengend Snippet: Ligand binding sites for 152 aa universal stress proteins of selected Bacillus cereus strains.

Article Snippet: The Invitrogen Oligo Perfect system was used to design primers to genes encoding two B. cereus USPs ZP04319786 and ZP04315896 as well as tuf gene, encoding elongation factor Tu.

Techniques: Ligand Binding Assay, Binding Assay

Journal: Bioinformatics and Biology Insights

Article Title: Functional Annotation Analytics of Bacillus Genomes Reveals Stress Responsive Acetate Utilization and Sulfate Uptake in the Biotechnologically Relevant Bacillus megaterium

doi: 10.4137/BBI.S7977

Figure Lengend Snippet: Confirmation of expression of genes for universal stress proteins in Bacillus cereus ATCC 10876. ( A ) Bands in the DNA gel demonstrate that both genes are expressed during the latter stages of exponential growth in Brain Heart Infusion broth. ( B ) Expression of tuf (elongation factor Tu) was used as a control to measure RNA isolation.

Article Snippet: The Invitrogen Oligo Perfect system was used to design primers to genes encoding two B. cereus USPs ZP04319786 and ZP04315896 as well as tuf gene, encoding elongation factor Tu.

Techniques: Expressing, Control, Isolation